Overall, these data are the first one to establish a timeline for axonal regeneration and synapse formation after injury in the olfactory system. It offers a number of advantages over other image-analysis software, including the availability and ease of use of custom-designed plugins for many different types of image analyses. In the olfactory bulb, we characterized the recovery of synaptic markers TH and synaptophysin after axons reached the olfactory bulb, where olfactory sensory neuron axons make synaptic contacts with dendrites of projection neurons. ImageJ is a Java-based program with a large community of developers and many resources. Data shows that after regeneration, neurons coexpress both markers 11 days after lesion. Analysis of maturation was done in the olfactory epithelium by studying the colocalization of the protein OMP and GFP. Using immunohistochemistry in combination with ImageJ software analysis, we are able to pinpoint the colocalization of markers of new olfactory sensory neurons (green fluorescent protein (GFP)) with markers of neuron maturation (olfactory marker protein (OMP)) and synapse formation (tyrosine hydroxylase (TH) and synaptophysin). This organization is an architectural layer housing ImageJ, ImageJ2, and their core extensions. ImageJ2 is a new version of ImageJ targeting scientific multidimensional image data. ImageJ is the original desktop application which has been in development since 1997. ImageJ.JS is an open source image processing web application designed for scientific multidimensional images steinr (Stein Rrvik) October 20, 2021, 3:48pm 9 Kinguin: when i try this macro i get an error: index (0) out of range in line 19 The size of arr2 is not defined in this macro, so you need to turn on the auto expandable array option. By using a fate mapping technique involving Cre-ERT2 mice, we are able to track the regeneration of these sensory neurons after a methimazole induced injury. ImageJ is open source software for processing and analyzing scientific images. Earlier studies have proven that the introduction of methimazole will effectively damage the olfactory sensory neurons while keeping the neural stem cells intact. In addition to the ImageJ forum, the ImageJ web site hosts a large selection of macros that not only provide useful functionality but also demonstrate how the different ImageJ command functions work. While the olfactory bulb has neurons that regenerate as well, we are interested in the regeneration that occurs in the olfactory epithelium after being injured because it possesses a source of neural stem cells – something unique to the rest of the body. This global network of ImageJ users is a great source of knowhow and advice to help get a novice user up and running. Processing of smell begins in the olfactory epithelium where sensory neurons are present and the olfactory bulb is the first stop in processing odor information in the central nervous system. The olfactory system is a great model to ask questions related to neuronal regeneration, axon guidance and synapse formation. I want to do this on sets of about 10-20 stacks: 1500x1500pixels, 500 slices, 1 channel in each stack.
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